Journal: Environmental science & technology

Article Title: Evaluating the Environmental Persistence and Inactivation of MS2 Bacteriophage and the Presumed Ebola Virus Surrogate Phi6 Using Low Concentration Hydrogen Peroxide Vapor

doi: 10.1021/acs.est.9b06034

Figure Lengend Snippet: Study Test Matrix

Article Snippet: Working stocks of each phage were prepared using a top agar overlay technique. ( 15 ) Briefly, agar plates using Tryptic Soy Agar with Magnesium for Phi6 (Hardy Diagnostics, Santa Clara, CA; Model G341) and LB Agar (Hardy Diagnostics, G77) for MS2 were inoculated with 100 μL (μL) of stock phage, 100 μL of bacterial culture ( Pseudomonas syringae LM2489 for Phi6 and Escherichia coli C-3000 [ATCC 15597] for MS2), and 5 mL (mL) of molten top agar (50 ± 5 °C) of the same media type.

Techniques:

Journal: Environmental science & technology

Article Title: Evaluating the Environmental Persistence and Inactivation of MS2 Bacteriophage and the Presumed Ebola Virus Surrogate Phi6 Using Low Concentration Hydrogen Peroxide Vapor

doi: 10.1021/acs.est.9b06034

Figure Lengend Snippet: Experimental Test Conditions

Article Snippet: Working stocks of each phage were prepared using a top agar overlay technique. ( 15 ) Briefly, agar plates using Tryptic Soy Agar with Magnesium for Phi6 (Hardy Diagnostics, Santa Clara, CA; Model G341) and LB Agar (Hardy Diagnostics, G77) for MS2 were inoculated with 100 μL (μL) of stock phage, 100 μL of bacterial culture ( Pseudomonas syringae LM2489 for Phi6 and Escherichia coli C-3000 [ATCC 15597] for MS2), and 5 mL (mL) of molten top agar (50 ± 5 °C) of the same media type.

Techniques: Concentration Assay

Journal: Environmental science & technology

Article Title: Evaluating the Environmental Persistence and Inactivation of MS2 Bacteriophage and the Presumed Ebola Virus Surrogate Phi6 Using Low Concentration Hydrogen Peroxide Vapor

doi: 10.1021/acs.est.9b06034

Figure Lengend Snippet: Decontamination efficacy results for Phi6 and MS2 diluted in blood, using low concentration hydrogen peroxide vapor (∼25 ppm), as average log reduction ±95% confidence interval limits.

Article Snippet: Working stocks of each phage were prepared using a top agar overlay technique. ( 15 ) Briefly, agar plates using Tryptic Soy Agar with Magnesium for Phi6 (Hardy Diagnostics, Santa Clara, CA; Model G341) and LB Agar (Hardy Diagnostics, G77) for MS2 were inoculated with 100 μL (μL) of stock phage, 100 μL of bacterial culture ( Pseudomonas syringae LM2489 for Phi6 and Escherichia coli C-3000 [ATCC 15597] for MS2), and 5 mL (mL) of molten top agar (50 ± 5 °C) of the same media type.

Techniques: Concentration Assay

Journal: Environmental science & technology

Article Title: Evaluating the Environmental Persistence and Inactivation of MS2 Bacteriophage and the Presumed Ebola Virus Surrogate Phi6 Using Low Concentration Hydrogen Peroxide Vapor

doi: 10.1021/acs.est.9b06034

Figure Lengend Snippet: Decontamination efficacy results for Phi6 and MS2 diluted in blood, using hydrogen peroxide vapor >400 ppm, as average log reduction ±95% confidence interval limits. Bars with an asterisk indicate no phage were recovered from any test coupons, and thus efficacy results are determined based on recovery from positive controls.

Article Snippet: Working stocks of each phage were prepared using a top agar overlay technique. ( 15 ) Briefly, agar plates using Tryptic Soy Agar with Magnesium for Phi6 (Hardy Diagnostics, Santa Clara, CA; Model G341) and LB Agar (Hardy Diagnostics, G77) for MS2 were inoculated with 100 μL (μL) of stock phage, 100 μL of bacterial culture ( Pseudomonas syringae LM2489 for Phi6 and Escherichia coli C-3000 [ATCC 15597] for MS2), and 5 mL (mL) of molten top agar (50 ± 5 °C) of the same media type.

Techniques:

Journal: Environmental science & technology

Article Title: Evaluating the Environmental Persistence and Inactivation of MS2 Bacteriophage and the Presumed Ebola Virus Surrogate Phi6 Using Low Concentration Hydrogen Peroxide Vapor

doi: 10.1021/acs.est.9b06034

Figure Lengend Snippet: Decontamination efficacy results for Phi6 and MS2 diluted in phosphate buffered saline, using low concentration hydrogen peroxide vapor (∼25 ppm), as average log reduction ±95% confidence interval limits. Bars with an asterisk indicate that no phages were recovered from any test coupons, and thus efficacy results are determined based on recovery from positive controls.

Article Snippet: Working stocks of each phage were prepared using a top agar overlay technique. ( 15 ) Briefly, agar plates using Tryptic Soy Agar with Magnesium for Phi6 (Hardy Diagnostics, Santa Clara, CA; Model G341) and LB Agar (Hardy Diagnostics, G77) for MS2 were inoculated with 100 μL (μL) of stock phage, 100 μL of bacterial culture ( Pseudomonas syringae LM2489 for Phi6 and Escherichia coli C-3000 [ATCC 15597] for MS2), and 5 mL (mL) of molten top agar (50 ± 5 °C) of the same media type.

Techniques: Saline, Concentration Assay